Down-regulation of transforming growth factor β1/activin receptor-like kinase 1 pathway gene expression by herbal compound 861 is related to deactivation of LX-2 cells

Figure 4 The level of Phosphorylated Smad1 with different treatment after 24 h. Western blot was used as described. A: Representative Western blot results of Phosphorylated Smad1. The positions of protein size markers were given; B: Densitometry of Western-blot analyzed by Gel-pro software. The levels of Phospho-Smad1 were normalized to the level of β-actin protein. Six independent experiments were performed. ^aP < 0.05 vs untreated LX-2 cell group, ^cP < 0.05 vs TGFβ1 treated group. Error bars, SD.