Control of gallbladder contractions by cholecystokinin through cholecystokinin-A receptors on gallbladder interstitial cells of cajal

Figure 2 Colocalization of CCKAR immunoreactivity with markers for ICC, kit in guinea pig gallbladder cultured ICC. CCK-AR immunoreactive ICC in the muscle of the gallbladder is distinctive of their labled processes. ICC possessed several processes (A-D); C-kit immunoreactive (green) ICC colocalize CCKAR (red); C: Compound micrographs from (A) and (B); D: Phase-contrast micrographs of the same gallbladder ICC at the same magnification; Often two or more labled ICC were aggregrated into a network (E-H), making it difficult to discern the individual cells; C-kit immunoreactive (green) ICCs colocalize CCKAR (red); G: Compound micrographs from (E) and (F); H: Phase-contrast micrographs of the same gallbladder ICCs at the same magnification; All these ICCs were big and had a large and angular nucleus; Bar (in D) 30 &mgr;m for (A-D) and (in (H)) 30 &mgr;m for (E-H).