Pseudomonas exotoxin antisense RNA selectively kills hepatitis B virus infected cells

Figure 3 RT-PCR of genomic DNA from HepG2 cells transfected with a HBV polymerase expression plasmid. 7.5 &mgr;g p77 RNA mixed with 7.5 &mgr;g p92 β-Gal expression plasmid were transfected in each experiment as well as plasmids expressing either wild-type or mutant HBV polymerase (7.5 &mgr;g each). Genomic DNA was extracted and analyzed by non-denaturing 2% agarose gel electrophoresis. PCR fragments indicate the presence of toxin p77 cDNA. A: Bio-Rad marker 50-2000 base pairs; B: PCR amplified fragment of HepG2 cells cotransfected with pCH391wt and r100; C: PCR amplified fragment of HepG2 cells cotransfected with pCH391mt and r100; D: P77 DNA as control DNA.