Relationship between expression of gastrin, somatostatin, Fas/FasL and caspases in large intestinal carcinoma

Table 1 Primers used for nested RT-PCR amplification of GAS and SS

Name	Primer sequence	PCR conditions	Size (bp)
GAS	1: 5’-TATGTGCTGATCTTTGCACTGGCT-3’ (sense: 6307-6330)	94°C, 30 s	282
	2: 5’-CTCATCCTCAGCACTGCGGCGGCC-3’ (antisense: 6718-6695)	60°C, 45 s
		72°C, 45 s
GAS	3: 5’-GAGCTACCCTGGCTGGAGCAGCAG-3’ (sense: 6415-6438)	94°C, 30 s	174
	2: 5’-CTCATCCTCAGCACTGCGGCGGCC-3’ (antisense: 6718-6695)	60°C, 45 s
		72°C, 45 s
SS	1: 5’-ATGCTGTCCTGCCGCCTCCAG-3’ (sense: 106-126)	94°C, 30 s	348
	2: 5’-ACAGGATGTGAAAGTCTTCCA-3’ (antisense: 1330-1310)	60°C, 45 s
SS	3: 5’-GCTGCTGCCGCGGGGAAGCAG-3’ (sense: 223-243)	94°C, 30 s	231
	2: 5’-ACAGGATGTGAAAGTCTTCCA-3’ (antisense: 1330-1310)	60°C, 45 s
		72°C, 45 s