Enhanced expressions and activations of leukotriene C4 synthesis enzymes in D-galactosamine/lipopolysaccharide-induced rat fulminant hepatic failure model

Figure 4 Immunohistochemical assay of the expression and localization of LTC4S (A) and mGST2 (B) in control and D-GalN/LPS-treated liver tissues. Immunohistochemical staining for paraffin-embedded liver sections from the control and D-GalN/LPS-treated rats was examined as described in Materials and Methods. Arrows indicate the representative positive cells expressing the brown granules. 1: Absence of staining on omission of primary antibody; 2: Control; 3-6: Groups treated with D-GalN/LPS at 1, 3, 6 and 12 h, respectively. Bar = 100 &mgr;m.