Transcriptional silencing of Dickkopf gene family by CpG island hypermethylation in human gastrointestinal cancer

doi:10.3748/wjg.14.2702

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May 7, 2008 (publication date) through Jul 2, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. May 7, 2008; 14(17): 2702-2714
Published online May 7, 2008. doi: 10.3748/wjg.14.2702

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Figure 5 Correlation of methylation in the promoter region with silencing of the Dkk genes in gastrointestinal cancer cell lines. RT-PCR and MSP were carried out using cDNA and genomic DNA from the indicated cancer cell lines, respectively. In vitro methylated DNA (CpG Genome Universal Methylated DNA from Chemicon International, Temecula, CA) was used as a positive control for methylated alleles, while DNA from normal lymphocytes was used as negative controls.

Citation: Maehata T, Taniguchi H, Yamamoto H, Nosho K, Adachi Y, Miyamoto N, Miyamoto C, Akutsu N, Yamaoka S, Itoh F. Transcriptional silencing of Dickkopf gene family by CpG island hypermethylation in human gastrointestinal cancer. World J Gastroenterol 2008; 14(17): 2702-2714
URL: https://www.wjgnet.com/1007-9327/full/v14/i17/2702.htm
DOI: https://dx.doi.org/10.3748/wjg.14.2702