Transcriptional silencing of Dickkopf gene family by CpG island hypermethylation in human gastrointestinal cancer

Figure 4 Scheme of the promoter regions of the Dkk genes and Krm2 gene. The 5' regions of the genes were identified by a BLAST search. We used a CpG island searcher that screens for CpG islands that meet the following criteria: CG percentage > 55% observed CpG/expected CpG > 0.65 length > 500 bp. CpG sites are represented by vertical bars. The arrows below CpG islands represent regions analyzed by MSP. The Dkk-1, Dkk-2, Dkk-3, and Krm2 genes contain CpG islands at their 5’ ends and Dkk-4 has a few CpGs in the promoter region.