Identification of osteopontin as the most consistently over-expressed gene in intrahepatic cholangiocarcinoma: Detection by oligonucleotide microarray and real-time PCR analysis

Figure 4 Comparison between expression values of osteopontin in 8 intrahepatic CCCs estimated by RT-PCR (LightCycler^®System; grey) and gene expression data (HU 133A, Affymetrix; white). The detected changes in osteopontin expression levels measured by RT-PCR reflected very well the changes in gene expression between tumor and non-malignant liver tissue obtained by microarray analysis. The results of RT-PCR revealed larger changes than the microarray data in all cases.