Identification of osteopontin as the most consistently over-expressed gene in intrahepatic cholangiocarcinoma: Detection by oligonucleotide microarray and real-time PCR analysis

Table 4 Expression levels of dysregulated genes in intrahepatic cholangiocarcinoma in relation to tumor differentiation according histopathological criteria (grading 1 to 3)

Probe set	Grading 1	Grading 2	Grading 3	Gene function
204857_at	3.09	4.14 (2.9-5.8)	No data	Hs. MAD1 (mitotic arrest deficient, yeast, homolog)-like 1
211571_s_at	3.49	0.98 (0.7-1.3)	1.411	mRNA for proteoglycan PG-M (V3), proteoglycan 2
209016_s_at	3.93	1.25 (0.3-6.7)	0.96 (0.92-1.0)	Hs. similar to keratin 7 (KRT7)
201325_s_at	4.27	4.5 (3.0-6.7)	1.08	Hs. epithelial membrane protein 1 (EMP1), mRNA
203779_s_at	5	No data	No data	Hs. epithelial V-like antigen 1 (EVA1), mRNA
211796	7.82	1.53 (1.1-1.9)	1.16	Hs. T cell receptor beta chain (TCRBV13S1-TCRBJ2S1) mRNA
204734_at	7.97	8.5 (6.3-11.4)	No data	Hs. keratin 15 (KRT15), mRNA
209126_x_at	10.26	8.254	No data	Hs. keratin 6 isoform K6f (KRT6F) mRNA
207935_s_at	19.94	10.4	No data	Hs. keratin 13 (KRT13), mRNA
217109_at	22.75	No data	No data	Hs. partial mRNA for sv7-MUC4 apomucin
205157_s_at	34.79	10.36	No data	Hs. keratin 17 (KRT17), mRNA
209070_s_at	No data	3.188	7.54 (6.9-8.2)	Hs. regulator of G-protein signaling 5, mRNA
204338_s_at	No data	18.98	128.5	Hs. regulator of G-protein signaling 4 (RGS4), mRNA
203638_s_at	No data	1.14 (0.7-2.3)	4.16 (2.7-6.4)	Hs. FGF receptor 2, keratinocyte growth factor receptor
208343_s_at	No data	1.36 (1.1-1.8)	2.45 (1.2-4.9)	Hs. CYP7A promoter binding factor mRNA, nuclear receptor 5
207846_at	No data	No data	1.414	Hs. POU domain, class 1, transcription factor 1 (Pit1)

No data: No significant (< 2-fold change) or consistent (< 70% of all cases) change in the expression level of the gene in this subgroup of CCC (G1-, G2- or G3-tumors).