Proliferation of L02 human hepatocytes in tolerized genetically immunocompetent rats

Figure 5 RT-PCR detection of human and rat albumin mRNA in liver tissue. 1: Standard molecular weight DNA marker; 2, 3: Normal rat liver tissue; 4, 5: Human liver tissue; 6, 7: Rats liver tissue with chimeric human hepatocytes at wk 2, 4, 6, 8, and mo 4, 6 in the Rts group and at wk 2, 4, 6, 8 in the 2AAF and the control group; 2, 4, 6: Rat albumin mRNA primers used in the RT-PCR reaction system, amplified 388 bp fragment of rat albumin mRNA presented in the rat liver tissue of 2 (normal rat liver tissue) and 6 (rats liver tissue with chimeric human hepatocytes), but not in 4 (human liver tissue); 3, 5, 7: Human albumin mRNA primers used in the RT-PCR reaction system, the amplified products did not present in 3 (normal rat liver tissue), but in 5 (human liver tissue) and 7 (rats liver tissue with chimeric human hepatocytes), amplified 241 bp human albumin mRNA presented in the rat liver tissue.