Inhibition of CXCR4 activity with AMD3100 decreases invasion of human colorectal cancer cells in vitro

Figure 4 A: Effect of AMD3100 on expression of MMP-2, MMP-9 and VEGF in SW480 cells. Protein samples extracted from SW480 cells treated for 26 h with AMD3100 were subjected to Western blotting for MMP-2, MMP-9, VEGF and GAPDH proteins. AMD3100 significantly decreased MMP-9 and VEGF protein expression in SW480 cells in a dose-dependent manner. The level of GAPDH expression was determined as a control for equivalent protein loading; B: RNA samples extracted from SW480 cells treated for 26 h with AMD3100 were subjected to RT-PCR for MMP-2, MMP-9, VEGF and β-actin. AMD3100 also significantly decreased expression of MMP-9 and VEGF mRNAs in SW480 cells, and the inhibitory effect was dose-dependent. RT-PCR for β-actin was performed in parallel to show an equal amount of total RNA in the sample.