Analysis of the human Atox 1 homologue in Wilson patients

Table 1 Oligonucleotide primers used to amplify Atox1

Exon	Sequence	Fragment size
Exon 1-Forward primer	5’-GGAGTGGGAGGGGCCTCCGGGACC-3’	273 bp
Exon 1-Reverse primer	5’-GTAAGCTAGGGGACAACAGCGGCTC-3’	273 bp
Exon 2 - Forward primer	5’-GCACTGTGTGGGGGTCACTCTACAG-3’	320 bp
Exon 2-Reverse primer	5’-GTGAGGATTAAATGATGTGATTCAC-3’	320 bp
Exon 3-Forward primer	5’-GAACTCTTTCTTGCTGTAACTGGGAG-3’	344 bp
Exon 3-Reverse primer	5’-GAGGGCTCTCCCGCTCCACTCAAG-3’	344 bp
Exon 4-Forward primer	5’-TGCAATGTCGCTATGTCCACACCA-3’	326 bp
Exon 4-Reverse primer	5’-GATCACACAGCAAAGAATCAGAATC-3’	326 bp