A red wine polyphenolic extract reduces the activation phenotype of cultured human liver myofibroblasts

Figure 2 Effect of the RWPE on expression of alpha-smooth muscle actin. A: Myofibroblasts were incubated for seven days in the absence of RWPE (A) or with 50 (B), 75 (C), or 100 (D) &mgr;g/mL RWPE. Aliquots of cell extracts grown in the same conditions were analyzed by Western blot (E) simultaneously for ASMA (A) and vimentin (V). F: The signals were quantified as described in Materials and Methods. The graph shows the mean of 2 separate experiments.