Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

Figure 3 Apoptotic DEF cells induced by NGVE virus infection stained with Annexin V-FITC/PI and observed under fluorescence microscope. The samples are analyzed for green fluorescence (FITC) and red fluorescence (PI). A: Different labeling patterns of the NGVE virus infected cells: early apoptotic cells, annexin V-FITC positive and PI negative (a and b); necrotic or late apoptotic cells, both annexin V-FITC and PI positive (c-f); dead cells, annexin V-FITC negative and PI positive (g and h); B: 72 h p.i., the early and late apoptotic cells.