Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

Figure 2 Flow cytometry of apoptotic DEF cells as assessed by annexin V-FITC fluorescent intensity. DEF cells are mock infected (A) and infected with NGVE virus (B). Cells harvested at 72 h p.i., and subsequently stained with annexin V-FITC/PI. One million cells are analyzed by flow cytometry, data are presented as fluorescent intensity units of annexin V-FITC (abscissa) and number of counts cells (ordinate). The M1 and M2 gates demarcate annexin V-FITC negative populations (non-apoptotic cells) and positive (apoptotic cells) populations.