Anti-sense oligonucleotide labeled with technetium-99m using hydrazinonictinamide derivative and N-hydroxysuccinimidyl S-acetylmercaptoacetyltriglycline: A comparison of radiochemical behaviors and biological properties

Table 3 Biodistribution of labeled compounds in BALB/c mice (% ID/g) (mean ± SD)

Tissue	0.5 h		1 h		2 h		4 h		Paired	t-test
	M	H	M	H	M	H	M	H
Blood	1.12 ± 0.76	6.21 ± 1.03	2.38 ± 0.63	6.56 ± 1.11	1.14 ± 0.42	3.58 ± 1.21	1.10 ± 0.09	2.83 ± 0.54	t = 4.347	P = 0.022
Heart	0.62 ± 0.31	2.13 ± 0.45	0.58 ± 0.07	1.64 ± 0.34	0.32 ± 0.05	1.16 ± 0.12	0.18 ± 0.08	0.81 ± 0.13	t = 5.362	P = 0.013
Lungs	3.11 ± 0.82	2.68 ± 0.65	3.87 ± 1.36	3.23 ± 1.04	3.04 ± 0.79	2.78 ± 1.03	2.08 ± 0.62	1.76 ± 0.36	t = -4.934	P = 0.016
Liver	7.52 ± 2.45	11.46 ± 2.31	13.19 ± 1.47	15.24 ± 2.53	9.21 ± 1.03	12.89 ± 1.68	9.48 ± 2.56	10.46 ± 1.97	t = 3.806	P = 0.032
Kidneys	11.42 ± 3.34	4.17 ± 1.05	17.13 ± 2.86	5.03 ± 0.94	24.58 ± 3.57	2.78 ± 0.68	21.95 ± 4.02	2.28 ± 0.95	t = -4.511	P = 0.020
Spleen	2.71 ± 1.62	4.87 ± 2.36	5.65 ± 0.93	6.08 ± 1.93	5.35 ± 0.26	4.08 ± 1.54	4.84 ± 1.33	5.21 ± 2.04	t = 0.603	P = 0.589
Stomach	1.08 ± 0.86	7.46 ± 2.13	2.58 ± 0.95	11.48 ± 3.01	1.73 ± 0.21	7.49 ± 1.86	1.41 ± 0.34	7.85 ± 3.02	t = 9.901	P = 0.002
Intestines	0.53 ± 0.31	1.26 ± 0.31	0.86 ± 0.14	2.68 ± 0.95	1.23 ± 0.19	6.44 ± 2.13	1.96 ± 0.53	7.84 ± 2.34	t = 2.706	P = 0.073
Muscle	1.35 ± 0.16	0.54 ± 0.21	0.87 ± 0.63	0.94 ± 0.81	0.75 ± 0.08	0.42 ± 0.06	0.64 ± 0.15	0.19 ± 0.05	t = -2.095	P = 0.127

M: ^99mTc-MAG₃-ASON; H: ^99mTc-HYNIC-ASON. Statistical analysis of the same tissue distribution of radioactivity was made after the injection of ^99mTc-HYNIC-ASON and ^99mTc-MAG₃-ASON.