Anti-sense oligonucleotide labeled with technetium-99m using hydrazinonictinamide derivative and N-hydroxysuccinimidyl S-acetylmercaptoacetyltriglycline: A comparison of radiochemical behaviors and biological properties

Table 1 Labeling efficacy and radiochemical purity of labeled compounds (mean ± SD)

	Interval between binding and labeling (d)			Radioactivity of 99mTcO4- (MBq)
	15	30	60	370	740	1480
Labeling efficiency (%)
Via HYNIC	57.36 ± 3.69	62.13 ± 4.25	62.87 ± 3.04	58.74 ± 5.32	62.86 ± 4.27	63.28 ± 3.38
Via NHS-MAG3	67.35 ± 4.03	68.35 ± 3.56	69.85 ± 4.63	68.67 ± 4.82	70.31 ± 5.09	71.56 ± 5.37
Radiochemical purity (%)
99mTc-HYNIC-ASON	95.75 ± 5.21	96.32 ± 4.92	95.86 ± 5.28	96.56 ± 4.45	96.87 ± 3.65	97.16 ± 4.34
99mTc-MAG3-ASON	96.43 ± 4.69	95.67 ± 5.17	96.39 ± 4.78	96.35 ± 6.12	95.86 ± 4.67	96.54 ± 5.65

ASON: Anti-sense oligonucleotide; HYNIC: Hydrazino nicotinamide derivative; NHS-MAG₃: N-hydroxysuccinimidyl S-acetylmercaptoacetyltriglycline.