Over-expressed and truncated midkines promote proliferation of BGC823 cells in vitro and tumor growth in vivo

Table 2 Frequency of tumorigenesis in nude mice

Injected cells	No. of mice	No. of days to tumor detection (percent of tumorigenesis)
		7	14	21	28
BGC823	7	0 (0.00)	3 (42.86)	6 (85.71)	7 (100)
BGC823/vector	7	0 (0.00)	4 (57.14)	7 (100)
BGC823/MK	7	5 (71.43)a	6 (85.71)a	7 (100)
BGC823/tMK	7	7 (100)b

P value was calculated by Fisher’s exact test. 7 d: BGC823/MK vs BGC823 or BGC823/vector, P = 0.0105; BGC823/tMK vs BGC823 or BGC823/vector, P = 0.0003. 14 d: BGC823/MK vs BGC823, P = 0.0174; BGC823/MK vs BGC823/vector, P = 0.0489.

^aP < 0.05;

^bP < 0.01.