Increased susceptibility for intrahepatic cholestasis of pregnancy and contraceptive-induced cholestasis in carriers of the 1331T>C polymorphism in the bile salt export pump

Table 1 Primers and probes of real-time PCR for allelic discrimination of ABCC2 SNPs in Caucasians

cDNA position1	SNP	Exon	Amino acid change	Eense-/antisense primer	Probes2
1249	G>A	10	V417I	5’-CCAACTTGGCCAGGAAGGA-3’/	VIC 5’-CTGTTTCTCCAACGGTGTA-3’
				5’-GGCATCCACAGACATCAGGTT-3’	FAM 5’-ACTGTTTCTCCAATGGTGTA-3’
3563	T>A	25	V1188E	5’-GCACCAGCAGCGATTTCTG-3’/	VIC 5’-ACACAATGAGGTGAGGAT-3’
				5’-AGGTGATCCAGGAAAAGACACATTT-3’	FAM 5’-ACAATGAGGAGAGGAT-3’
4544	G>A	32	C1515Y	5’-GTAATGGTCCTAGACAACGGGAAG-3’/	VIC 5’-AGAGTGCGGCAGCC-3’
				5’-CCAGGGATTTGTAGCAGTTCTTCAG-3’	FAM 5’-ATTATAGAGTACGGCAGCC-3’

¹cDNA sequence from GenBank accession numbers NM_000392 starting at the ATG;

²For each SNP two probes were designed and labeled with the fluorescent reporter dyes VIC (allele 1) and FAM (allele 2). SNP: Single nucleotide polymorphism.