Selective COX-2 inhibitor, NS-398, suppresses cellular proliferation in human hepatocellular carcinoma cell lines via cell cycle arrest

Figure 5 Inhibition of PCNA expression by NS-398. A: Down-regulation of PCNA expression in both cell lines after NS-398 treatment. Cells were treated for 24, 48, and 72 h with 100 μmol/L NS-398. Controls were treated with an equivalent volume of DMSO without NS-398. PCNA expressions in HepG2 and Huh7 cells were inhibited in a time-dependent (as shown above) and dose-independent (data not shown) manner; B: Quantification of the relative amount of each band by image analysis.