Selective COX-2 inhibitor, NS-398, suppresses cellular proliferation in human hepatocellular carcinoma cell lines via cell cycle arrest

Figure 3 Flow cytometric analysis after exposure of HepG2 (A) and Huh7 (B) cells to NS-398. Cells were treated with 100 μmol/L NS-398 for 24, 48, and 72 h, stained with annexin V-FITC and propidium iodide and analyzed on a FACScalibur flow cytometer. Quadrants 2-4 represent secondary necrotic cells, viable cells, and apoptotic cells, respectively.