Selective COX-2 inhibitor, NS-398, suppresses cellular proliferation in human hepatocellular carcinoma cell lines via cell cycle arrest

Figure 2 Proliferation of HepG2 and Huh7 cells inhibited by NS-398. A: Time-dependent growth-inhibitory effect of NS-398. One hundred μmol/L NS-398 was added to HepG2 and Huh7 cells, which were cultured for 24, 48 or 72 h, and subjected to WST-1 assay. Absorbance was detected at 450 nm with an ELISA plate reader, and the growth rates of both cell lines treated with NS-398 were reduced compared to controls. Control cells were treated with an equivalent volume of DMSO without NS-398, respectively; B: Dose-dependent growth-inhibitory effect of NS-398. Cells treated with various concentrations of NS-398 were cultured for 72 h, and quantified by WST-1 assay. Data from 3 independent experiments are shown as e mean ± SD (^aP < 0.05).