Selective COX-2 inhibitor, NS-398, suppresses cellular proliferation in human hepatocellular carcinoma cell lines via cell cycle arrest

Figure 1 Immunoblot analysis for COX-2 protein levels in HepG2 and Huh7 cells. Cells were treated with 100 μmol/L NS-398 for 0, 24, 48 and 72 h. Control cells were treated with DMSO without NS-398. The upper bands represent 72 kDa COX-2-specific staining while the lower bands are β-actin controls. COX-2 expression was detectable but low in both cell lines. Decreased changes were observed in COX-2 expression with increasing time.