Role of CARD15, DLG5 and OCTN genes polymorphisms in children with inflammatory bowel diseases

Table 1 Primers sequences, methodology, and restriction enzymes used for genotyping

Gene locus	SNPs		Sequence	Methods/enzyme
CARD15	R702W	For	5’ACCTTCAGATCACAGCAGCC3’	DHPLC
		Rev	5’GCTCCCCCATACCTGAAC3’
	G908R	For	5’AAGTCTGTAATGTAAAGCCAC3’	RFLP/Hha I
		Rev	5’CCCAGCTCCTCCCTCTTC3’
	L1007fsinsC	For	5’CTCACCATTGTATCTTCTTTTC3’	DHPLC
		Rev	5’GAATGTCAGAATCAGAAGGG3’
IBD5	IGR2198a_1	For	5’GGGGCAATTCTATGAGGACA3’	RFLP/Nla III
		Rev	5’CCAGAGACACTGGGACATCA3’
	IGR2096a_1	For	5’GTAGCGAGAGGCTCCACAGT3’	RFLP/Dra I
		Rev	5’TCCTCCATGCTACTGCTCTG3’
OCTN1	C1672T	For	5’GGGTAGTCTGACTGTCCTGATTG3’	TaqMan
SLC22A4		Rev	5’TCTGGAAGAGTCATTCCCAAACTTTC3’
		VIC	5’AAGGGTGAGGATTC3’
		FAM	5’AAGGGTGAAGATTC3’
OCTN2	G207C	For	5’CCGCTCTGCCTGCCA3’	TaqMan
SLC22A5		Rev	5’GCGGCTGGCCTTACATAGG3’
		VIC	5’CAGGCCCGGAACC3’
		FAM	5’CAGGCCCGCAACC3’
DLG5	R30Q		C_7432738_10	TaqMan

DHPLC: Denaturing high performance liquid chromatography; RFLP: Restriction fragment length polymorphisms; TaqMan: ABI Prism 7700 sequencer detector.