Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells

Figure 4 Ceramide inhibited activation of ERK pathway in Bel7402. Bel7402 cells were incubated with different concentrations of ceramide as indicated. Total proteins were extracted and resolved on SDS-PAGE. Phospho-ERK1/2 and c-myc were normalized to total Erk1/2 and internal standard actin was determined by Western blot. Data represents the results of three separate experiments.