Evidence for a sequential transfer of iron amongst ferritin, transferrin and transferrin receptor during duodenal absorption of iron in rat and human

Figure 5 Microautoradiography and immunohistocheminstry with transferrin and transferrin receptor demonstrates the in vivo uptake of iron through transferrin receptor. A: Immunohistochemical localization of transferrin left panel (Tf) and transferrin receptor right panel (TfR) in the duodenal sections of intestine of iron deficient rat (ID); B: transferrin stain in iron over loaded rat (top panel) (OL)at basal status and tranferrin receptor on the surface of the epithelial cells showing no internalization(bottom panel) in iron overloaded condition after oral administration of radioactive iron (10X); C: Co-localization of iron, transferrin and transferrin receptors in the duodenal sections of intestines of iron deficient rats obtained 15 min after oral administration of radioactive iron. Microautoradiographic localization of iron immunohistochemical localization of transferrin transferring receptor.