Role of soluble factors and three-dimensional culture in in vitro differentiation of intestinal macrophages

Figure 7 Western-blot for caspase-3 of mixed spheroids after 24 h and three days. Mixed spheroids of IEC and MO, control cells and MO or control spheroids generated only from IEC or control cells were disaggregated after 24 h and three days of culture. Western-blots for activated caspase-3 were performed. Lane 1: HT-29 without MO 24 h; lane 2: HT-29/MO (1:1) 24 h; lane 3: J82 without MO 24 h; lane 4: J82/MO (1:1) 24 h; lane 5: HT-29 without MO 3 d; lane 6: HT-29/MO (1:1) 3 d; lane 7: J82 without MO 3 d; lane 8: J82/MO (1:1) 3 d; lane 9: positive control for activated caspase-3. No activated caspase-3 could be detected in co-cultures of MO and IEC or in spheroids generated only from IEC.