Role of soluble factors and three-dimensional culture in in vitro differentiation of intestinal macrophages

Figure 1 Immunohistochemical detection of MO/MAC antigen expression after 7 d of culture in IEC conditioned medium. Freshly elutriated MO were incubated in unconditioned control media, conditioned media of the IEC lines HT-29 and WiDr and conditioned medium of the control cell line J82 of non-intestinal origin for seven days. Antigen expression was determined by immunohistochemistry (APAAP-method). Expression of the MO/MAC specific antigens CD14, CD16, CD11b and CD11c was determined. All tested antigens were detectable on the cells after the seven-day culture period. Incubation in IEC-conditioned medium had no influence on antigen expression.