Embryonic stem cells develop into hepatocytes after intrasplenic transplantation in CCl4-treated mice

Figure 3 Disappearing GFP-immuno-positivity. GFP-immuno-positivity on PD 10, 20, and 30 was examined using liver specimens from group 1 mice. A: GFP-immunoreactivity was clearly observed in the periportal regions on PD 10; B: GFP-immunoreactivity was still detected on PD 20, though the intensity was weaker than on PD 10. Cells presenting GFP-immunoreactivity were more inwardly distributed in the hepatic lobules, as compared to PD 10; C: Scant or no GFP-immuno-positivity was detected on PD 30; D: The average numbers of GFP-positive cells around the portal vein were determined after examining 10 microscopic fields in the periportal area at 400 x magnification.