Embryonic stem cells develop into hepatocytes after intrasplenic transplantation in CCl4-treated mice

Figure 1 Grouping of experimental animals. Female C57BL/6 mice were divided into 3 groups. Intraperitoneal injection of CCl₄ at 0.5 mL/kg body weight was performed twice a week in group 1 mice (n = 12) and group 3 mice (n = 6), while 0.2 mL of saline was injected instead of CCl₄ in group 2 mice (n = 12). One day after the second intraperitoneal injection of CCl₄ or saline, 1 x 10⁵ GFP-positive undifferentiated ES cells (0.1 mL of 1 x 10⁶/mL solution) were transplanted into the spleens in groups 1 and 2, whereas no cell transplantation was performed with group 3.