Detection of aberrant methylation in fecal DNA as a molecular screening tool for colorectal cancer and precancerous lesions

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Feb 14, 2007 (publication date) through Nov 30, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 14, 2007; 13(6): 950-954
Published online Feb 14, 2007. doi: 10.3748/wjg.v13.i6.950

Table 2 Primers for MSP

Primer	Sequences (5’-3’)	Annealingtemperature	Productsize (bp)
SRFP2-MF	GGGTCGGAGTTTTTCGGAGTTGCGC	62°C	138
SRFP2-MR	CCGCTCTCTTCGCTAAATACGACTCG
SRFP2-UF	TTTTGGGTTGGAGTTTTTTGGAGTTGTGT	58°C	145
SRFP2-UR	AACCCACTCTCTTCACTAAATACAACTCA
HPP1-MF	TTTAGCGGACGATTTTTTCGTTTCG	57°C	122
HPP1-MR	AACGACGACGATAACAATAA
HPP1-UF	TTTAGTGGATGATTTTTTTGTTTTG	57°C	122
HPP1-UR	AACAACAACAATAACAATAA
MGMT-MF	TTTCGACGTTCGTAGGTTTTCGC	56°C	81
MGMT-MR	GCACTCTTCCGAAAACGAAACG
MGMT-UF	TTTGTGTTTTGATGTTTGTAGGTTTTTGT	59°C	93
MGMT-UR	AACTCCACACTCTTCCAAAAACAAAACA

U: unmethylated; M: methylated; F: forward; R: reverse.

Citation: Huang ZH, Li LH, Yang F, Wang JF. Detection of aberrant methylation in fecal DNA as a molecular screening tool for colorectal cancer and precancerous lesions. World J Gastroenterol 2007; 13(6): 950-954
URL: https://www.wjgnet.com/1007-9327/full/v13/i6/950.htm
DOI: https://dx.doi.org/10.3748/wjg.v13.i6.950