Soluble forms of extracellular cytokeratin 18 may differentiate simple steatosis from nonalcoholic steatohepatitis

Table 5 Diagnostic performance of serum M30-antigen and M65-antigen levels for the diagnosis of definitive NASH

Patientcategory	Serumbiomarker	Cut-off value1(IU/L)	Sensitivity(95% CI)	Specificity(95% CI)	Positive predictivevalue (%)	Negative predictivevalue (%)	Negativelikelihood ratio	Area under theROC curve (95% CI)
Borderline NASH	M30-antigen	121.6	60 (44.3-74.3)	95.8 (78.8-99.3)	96.40	56.10	0.42	0.783 (0.668-0.783)
	M65-antigen	241.34	68.9 (53.3-81.8)	83.3 (62.6-95.2)	88.60	58.80	0.37	0.809 (0.697-0.894)
Simple steatosis	M30-antigen	109.64	62.2 (46.5-76.2)	100 (66.2-100.0)	100.00	34.60	0.38	0.83 (0.703-0.918)
	M65-antigen	186.77	93.3 (81.7-98.5)	66.7 (30.1-92.1)	93.30	66.70	0.10	0.807 (0.677-0.902)
Normal tissue	M30-antigen	96.5	64.4 (48.8-78.1)	100 (48.0-100.0)	100.00	23.80	0.36	0.724 (0.508-0.841)
	M65-antigen	227.18	73.3 (58.1-85.4)	80 (28.8-96.7)	97.10	25.00	0.33	0.809 (0.673-0.906)
Healthy controls	M30-antigen	82.52	75.6 (60.5-87.1)	85.7 (72.7-94.0)	82.90	79.20	0.29	0.881 (0.798-0.938)
	M65-antigen	189.64	91.4 (77.6-98.6)	68.1 (36.1-94.2)	92.00	68.20	0.18	0.94 (0.839-0.980)
No-NASH	M30-antigen	121.6	60 (44.3-74.3)	97.4 (86.1-99.6)	96.40	67.30	0.41	0.787 (0.683-0.869)
	M65-antigen	243.82	68.9 (53.3-81.8)	81.6 (65.7-92.2)	81.60	68.90	0.38	0.809 (0.708-0.887)

¹Cut-off value corresponding to the highest accuracy value (minimal false negative and false positive results).