p53-expressing conditionally replicative adenovirus CNHK500-p53 against hepatocellular carcinoma in vitro

Figure 5 Oncolytic efficacy induced by CNHK500-p53 infection evaluated by MTT assay. Statistical analysis was performed using Student’s t test for differences among groups. Statistical significance was defined as P < 0.05. A: HCC cell lines HepG2, Bel-7402 and normal cell lines BJ, MRC-5 were infected at different MOI of 0.001-100 pfu/cell of CNHK500-p53. Cell viability was measured by MTT assay 7 d after infection. CNHK500-p53 induced a more powerful oncolysis in HCC cell lines than in normal cell lines (P < 0.05); B: Seven days after infection with virus, CNHK500-p53 not only showed more powerful cytolysis than CNHK500 and WtAd5 in HepG2, but also less profound cytolysis than WtAd5 in BJ (P < 0.05); C: IC50 of CNHK500-p53, CNHk500, and WtAd5 demonstrated significant differences among groups with ^a,c,eP < 0.05.