p53-expressing conditionally replicative adenovirus CNHK500-p53 against hepatocellular carcinoma in vitro

Figure 4 Cytopathic effects associated with CNHK500-p53, CNHK500, Ad-p53, and Ad-Blank infection in HCC cell lines HepG2 (A), Hep3B (B), Bel-7402 (C), and normal cell line BJ (D). Seven days after virus infection, all the cells were stained with crystal violet and photographed. Comparison with other viruses, CNHK500-p53 showed the strongest selective cytolysis against HCC cell lines. Infection with CNHK500-p53 at MOI of 0.1-1 was sufficient to induce its lytic effects in Bel-7402, HepG2, and Hep3B, although the sensitivity varied among the cell types. In contrast, no apparent cytopathic effects were observed in BJ even at MOI of 100 after CNHK500-p53 infection, which was similar to other viruses.