p53-expressing conditionally replicative adenovirus CNHK500-p53 against hepatocellular carcinoma in vitro

Figure 1 Schematic diagram of the CNHK500-p53 adenoviral construct. A 310-bp fragment of human telomerase reverse transcriptase (hTERT) promoter with three E-boxes (CACGTG) downstream of the core sequence replaced the endogenous E1A promoter (digested with NotI and XhoI) to control the expression of E1A. A 241-bp fragment of hypoxia response element (HRE) promoter replaced the endogenous E1B promoter to control the expression of E1B. A 1805-bp fragment of transgene expression cassette containing cytomegalovirus (CMV) promoter + p53 + SV40 poly A was inserted into the downstream of E1A (digested with AgeI + NotI) to generate CNHK500-p53. ITR, inverted terminal repeat; ψ, the adenovirus 5 packing signal.