Acanthus ilicifolius plant extract prevents DNA alterations in a transplantable Ehrlich ascites carcinoma-bearing murine model

Figure 4 Light micrographs of tissue sections from murine liver (after 30 d of tumour transplantation) showing immunostaining of metallothionein (MT) with anti-rat MT-1 antibody and 3, 3'-diaminobenzidine tetrahydrochloride (DAB). A and B: Strong immunostaining of MT protein in EAC Control mice; C: Reduced immunostaining of MT in EAC + ALE-treatment mice. ALE: Aqueous leaf extract of A. ilicifolius; EAC: Ehrlich ascites carcinoma. Arrow Head ( ) indicates intense immunostaining of MT protein with prominent focal expression and isolated clusters of MT-positive cells. Arrow (↑) indicates scattered/individual MT immunopositive cells. Magnification, A: × 100; B and C: × 270.