Antitumor and antiangiogenic activities of anti-vascular endothelial growth factor hairpin ribozyme in human hepatocellular carcinoma cell cultures and xenografts

Figure 1 Construction and identification of recombinant eukaryotic expression plasmid pcDNA3. 1+/RZ. A: Sequencing result of the anti-hVEGF hairpin ribozyme gene subcloned into pcDNA3.1+; B: Integration of the ribozyme gene detected by PCR (1: SMMC-7721 cells; 2: SMMC-7721/pcDNA3.1+cells; 3: SMMC-7721/RZ cells; M: DL2000 marker); C: Transcription of the ribozyme gene detected by RT-PCR (1: SMMC-7721/RZ cells; 2: SMMC-772 cells; 3: SMMC-7721/ pcDNA3.1+ cells; M: DL2000 marker).