Clinical Research
Copyright ©2007 Baishideng Publishing Group Inc.
World J Gastroenterol. Dec 21, 2007; 13(47): 6396-6403
Published online Dec 21, 2007. doi: 10.3748/wjg.v13.i47.6396
Table 1 The primer sequence of the p16 genes
ExonPrimer sequenceSize(bp)Anneling temperature (°C)
Exon1αF: 5'-GAGAGGGGGAGAGCAGGCAG-3'25058
R: 5'-GCAAACTTCGTCCTCCAGAGT-3'
Exon2-1F: 5'-AGCTTCCTTTCCGTCATGC-3'20356
R: 5'-GCAGCACCACCAGCGTG-3'
Exon2-2F: 5'-AGCCCAACTGCGCCGAC-3'14758
R: 5'-CCAGGTCCACGGGCAGA-3'
Exon2-3F: 5'-TGGACGTGCGCGATGC-3'18956
R: 5'-GGAAGCTCTCAGGGTACAAATTC-3'
Exon3F: 5'-CCGGTAGGGACGGCAAGAGA-3'16958
R: 5'-CTGTAGGACCCTCGGTGACTGATGA-3'