Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes: Implications in intestinal pathophysiology

Table 3 Primers used for RT-PCR

Gene symbol	Gene ID (NCBI)	Gene name	Gene role	Primer	Primer sequence 5’-3’
BMF	90427	Bcl2 modifying factor, transcript variant 1	Has a single Bcl2 homology domain 3 (BH3), binds Bclk2 proteins and functions as an apoptotic activator	F	GCTTCAGTTGCATTGCAGACCAGTT
				R	AGAGCCCTTGGGAATTCTCACCAT
CD248	57124	CD248 antigen = endosialin	A gene regulated by the cell density in vitro. Has a calcium binding domain	F	TCAACTACGTTGGTGGCTTCGAGT
				R	AGTTGGGATAATGGGAAGCTGGGT
PPM1E	22843	Protein phosphatase 1E	Member of the PP2C family of Ser/Thr phosphatases known to be negative regulators of stress response pathways	F	ATGCCTCCATTCACCTCCACGTTA
				R	TGTCATAGAAGCCATCACAGGCCA
FXYD3	5349	FXY domain containing ion transport reg. 3	The protein encoded by this gene may function as a chloride channel or as a chloride channel regulator	F	AATGCAAGTTTGGCCAGAAGTCCG
				R	TTGCATATGAGGTCCCATGGCTGA
OAS2	4939	2’-5’-oligoadenylate synthetase 2	This enzyme family plays a significant role in the inhibition of cellular protein synthesis	F	AGAAGCCAACGTGACATCCTCGAT
				R	TGCTGGAGTTCAGTGAAGCAGACT
FY	2532	Duffy blood group antigen	Helps in leukocyte recruitment to sites of inflammation by facilitating movement of chemokines across the endothelium	F	TGACTCTGCACTGCCCTTCTTCAT
				R	TTGACAACAGCAACAGCTTGGACC
CERK	64781	Ceramide kinase	Integral to membranes, has roles in arachidonic acid release and production of eicosanoids	F	TGAGAAGAAACGGTGGTTGGGTCT
				R	AGCATTTCCGGATGAGGATGAGGT
HPSE	10855	Heparanase	Cell surface expression and secretion markedly promote tumor angiogenesis and metastasis	F	ACCTTTGCAGCTGGCTTTATGTGG
				R	CTTGCACGCTTGCCATTAACACCT
GAPDH	2597	Glyceraldehyde-3-phosphate dehydrogenase	Used as reference	F	GACCACAGTCCATGCCATCAC
				R	GAGCTTCAGAAAGTGGTCGTTGA