Effect and mechanism of β-L-D4A on DNA polymerase α

Figure 1 A280 and gradient concentration of KPO₄ in each chromatography. The curve with spots is for A280, the other is for KPO₄. A: A broad peak of protein from the 16^th tube (0.115 mol/L) to the 90^th tube (0.185 mol/L), clearly observed in the second DE52 chromatography; B: Phosphocellulose chromatography displaying a major peak of protein identified at 0.165 mol/L KPO₄ and a minor trailing shoulder at 0.21 mol/L; C: Hydroxylapatite chromatography showing a single sharp peak of protein at 0.085 mol/L KPO₄; D: DNA-cellulose chromatography revealing a single sharp peak at 0.085 mol/L KPO₄.