Moving forward in colorectal cancer research, what proteomics has to tell

Figure 3 Representation of the two antibody microarray experimental formats. Direct labelling: single-capture antibody experiments; all proteins in a sample are labelled (black circles) thereby providing a means for detecting bound proteins following incubation on an antibody microarray. Dual-antibody (capture and read-out antibody) sandwich immunoassays: proteins captured on an antibody microarray are detected by a cocktail of tagged detection antibodies, which are matched to the spotted antibodies. The detector antibody tag is then measured by binding of a labelled (empty circles) read-out antibody.