Silencing SMYD3 in hepatoma demethylates RIZI promoter induces apoptosis and inhibits cell proliferation and migration

Figure 2 RNAi specifically inhibits SMYD3 expression in HCC cell lines. A: Schematic drawing of the Pgenesil-1 vector; B: The predicted secondary structures of the Pgenesil-1-s1 and Pgenesil-1-s2 transcripts target SMYD3 are shown; C: 48h after transfection, Western blot analysis showed the inhibitory effect of plasmids expressing SMYD3 shRNAs in HepG2 and SMMC-7721 cells.