Constitutive androstane receptor agonist, TCPOBOP, attenuates steatohepatitis in the methionine choline-deficient diet-fed mouse

Figure 1 Over-expression of CAR target genes in MCD diet-fed mice treated with TCPOBOP. CAR activation was assessed by measuring CYP2B10 and CYP3A11 (CAR target genes) expression in whole liver from vehicle-treated and TCPOBOP-treated chow-fed and MCD diet-fed mice. Expression was measured by real-time PCR and normalized as a ratio using 18S mRNA as housekeeping genes. A value of 1 for this ratio was arbitrarily assigned to the data obtained from vehicle-treated CAR^+/+ mice. (A) CYP2B10 and (B) CYP3A11 mRNA expressions were increased in TCPOBOP-treated (3 mg/kg ip for 3 d) chow-fed mice (^bP < 0.01 for CYP2B10 and CYP3A11 compared to control) and MCD diet-fed mice (^dP < 0.01 for CYP2B10 and CYP3A11 compared to untreated). This phenomenon was abated by treatment with a CAR inhibitor androstanol (100 mg/kg ip) daily for 3 d at the starting of weeks one and two of the MCD diet (total of 6 injections), (n = 5 in each group).