Chinese medicinal compound delisheng has satisfactory anti-tumor activity, and is associated with up-regulation of endostatin in human hepatocellular carcinoma cell line HepG2 in three-dimensional culture

Figure 8 Immunohistochemical staining patterns of formalin-fixed and paraffin-embedded multicellular spheroids of HepG2 cells (× 200). [A: delisheng; B: adriamycin; C: 5-fluorouracil; D: negative control PBS; E: negative control (Primary antibody was substituted by normal rabbit serum)]. Endostatin expression was confirmed in multicellular spheroids, and the expression of endostatin with treatment of delisheng was higher than that of 5-fluorouracil, adriamycin and PBS (P < 0.05).