Effects and mechanisms of silibinin on human hepatoma cell lines

Figure 2 Silibinin's effects on proliferation of HCC cells. After HuH7 cells were treated with silibinin at IC₂₅ and IC₅₀ doses, immunoprecipitation (IP) and immunoblots (IB) were performed in triplicate for each specimen. A mean densitometer reading was expressed in the respective box and used for statistical analysis. A: β-actin for internal control; B: Ki-67; C: CD1/CDK4 complex; D: p-Rb; E: E2F1-DP1 complex; F: p21^Waf1/Cip1; G: p27^Kip1; H: p21^Waf1/Cip/CDK4 complex; I: p27^Kip1/CDK4 complex. ^aP < 0.05, ^bP < 0.01, vs control.