Serum-free culture of H pylori intensifies cytotoxicity

Figure 5 Analysis of the extract cultured without serum. Cell viability that represents the putative cytotoxic factor was measured for cell viability at A₄₁₅ using the WST1 method. Column eluates that represent protein densities were monitored at A₂₈₀ for each filtrated fraction. A: Cation exchange chromatography of the cell extract protein approximately 100 passages (approximately one year) after the onset of culturing without serum. The cell extract proteins were eluted with a stepwise gradient of 0-0.5 mol/L NaCl; B: Size exclusion chromatography of the cell extract protein approximately 100 passages (approximately one year) after the onset of culturing without serum.