Serum-free culture of H pylori intensifies cytotoxicity

Figure 2 Morphologic change of HeLa cells caused by H pylori 1. 0 mg/mL cell extract with or without serum. HeLa cells exposed to the extract cultured without serum showed the characteristic morphological changes in 12 h. A: HeLa cells exposed to the extract cultured with serum 6 h and 12 h after addition of the extract showed no remarkable changes but a small decrease in cell number; B: HeLa cells exposed to the extract cultured without serum 6 h and 12 h after addition of the extract showed the round-formed shape or round debris with a decayed contour through the elongated or spindle-like shape without vacuolations. Scale bar, 200 μm.