Nerve growth factor involvement in liver cirrhosis and hepatocellular carcinoma

Figure 6 NGF distribution in cirrhotic tissue from patient with HCC by immunogold labelling. A, B: Transmission electron micrographs of hepatocytes showing positive immunogold reaction on cytoplasmic vesicles (black arrows), free in the cytoplasm (double pointed arrows) and along endoplasmic reticulum (white arrows); C, C₁, C₂: Endothelial cells; positive immunogold reaction on cytoplasmic vesicles (black arrows) and free in the cytoplasm (double pointed arrows); D, D₁: Spindle-shaped cells; E, D₁: Biliary epithelial cells. In C₁, C₂, D₁ and E₁ details at higher magnification are shown. hep: hepatocytes; cf: collagen fibers; ER: endoplasmic reticulum. Scale bars = A, C, D, E: 2 μm; B, C₁, C₂, D₁, E₁: 100 nm.