Nerve growth factor involvement in liver cirrhosis and hepatocellular carcinoma

Figure 2 NGF distribution in HCC tissue. E, F, G, I, J, K: Confocal microscopy images of immuno-stained sections showing NGF immunoreaction (red hue) in the cytoplasm (F) and on nuclei (I) of some hepatocytes, on endothelial cells, on lymphocytes and on Kupffer cells; no immunoreaction was observed in controls performed by substitution of primary antisera with non-immune rabbit serum. A: Images, at two different magnification, of H&E stained sections; B, C, D, H: Images of semithin sections from samples embedded in Spurr epoxy resin. Differently coloured arrows indicate the different cell types (see legend). n: nucleus; hep: hepatocyte; bvl: blood vessel lumen.