Three novel missense germline mutations in different exons of MSH6 gene in Chinese hereditary non-polyposis colorectal cancer families

Table 1 Primer sequences and PCR condition of different exons of MSH6 gene

EN	Primer sequence (5’-3’)	Size(bp)	AT(°C)	CN(C)
Exon1	M13F-AGCTCCGTCCGACAGAAC	381	68	38
	M13R-CTGTGCGAGCCTCCCCT
Exon2	M13F-TGCCAGAAGACTTGGAATTG	325	63	32
	M13R-CAAACACACACACATGGCAG
Exon3	M13F-GATGGGGTTTGCTATGTTGC	341	67	36
	M13R-TACACCCTCCCCCTTTCTTC
Exon4.1	M13F-GGCTGCACGGGTACCATTAT	390	60	34
	M13R-CATTCTCTTCCGCTTTCGAG
Exon4.2	M13F-GCCAGACACTAAGGAGGAAGG	386	59	35
	M13R-TAGATGCATCAAAATCGGGG
Exon4.3	M13F-TGGCTTAAGGAGGAAAAGAGA	378	60	34
	M13R-TCTACATCGTGCCTCCATCA
Exon4.4	M13F-TTCTGGCTTTCCTGAAATTG	374	60	34
	M13R-TAAATCTCGAACAATGGCGA
Exon4.5	M13F-TCTGGCCATACTCGTGCATA	329	60	34
	M13R-AGCACCTGGGGTAACATCAC
Exon4.6	M13F-TCAGGAAGGTCTGATACCCG	353	62	33
	M13R-GCACCATTCGTTGATAGGCT
Exon4.7	M13F-AAGTGAATTGGCCCTCTCTG	483	62	30
	M13R-TGGTTCTGACTCTTCAGGGG
Exon4.8	M13F-TTTTGGTAAGCGGCTCCTAA	465	62	35
	M13R-TTTCGAGCCTTTTCATGGTC
Exon4.9	M13F-TTTCTGCTCTGGAAGGATTC	440	62	30
	M13R-TCGTTTACAGCCCTTCTTGG
Exon4.10	M13F-TGAACAGAGCCTCCTGGAAT	390	62	32
	M13R-CAGCTGGCAAACAGCACTAC
Exon5	M13F-CTGATAAAACCCCCAAACGA	403	62	30
	M13R-CTGTGTTTGGAAAATGATCACC
Exon6	M13F-CCAGTCATAAAAGACCTTTTCC	192	56	34
	M13R-GACTGAATGAGAACTTAAGTGGG
Exon7	M13F-AAGGTGAAAGTACATTT	119	61	33
	M13R-TTCAAATGAGAAGTTTAATG
Exon8/9	M13F-CCTTTGAGTTACTTCCTT	573	61	32
	M13R-TCATAGTGCATCATCCCTTCC
Exon10	M13F-GGAAGGGATGATGCACTATG	254	61	35
	M13R-AAGAAAATGGAAAAATGGTCA

Abbreviation: EN, Exon name. AT, Anneal temperature. CN, Cycle number.The sequence of M13F was 5’-GTAAAACGACGGCCAGT-3’. The sequence of M13R was 5’-AACAGCTATGACCATG-3’.