Rapid Communication
Copyright ©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Oct 7, 2007; 13(37): 5021-5024
Published online Oct 7, 2007. doi: 10.3748/wjg.v13.i37.5021
Table 1 Primer sequences and PCR condition of different exons of MSH6 gene
ENPrimer sequence (5’-3’)Size(bp)AT(°C)CN(C)
Exon1M13F-AGCTCCGTCCGACAGAAC3816838
M13R-CTGTGCGAGCCTCCCCT
Exon2M13F-TGCCAGAAGACTTGGAATTG3256332
M13R-CAAACACACACACATGGCAG
Exon3M13F-GATGGGGTTTGCTATGTTGC3416736
M13R-TACACCCTCCCCCTTTCTTC
Exon4.1M13F-GGCTGCACGGGTACCATTAT3906034
M13R-CATTCTCTTCCGCTTTCGAG
Exon4.2M13F-GCCAGACACTAAGGAGGAAGG3865935
M13R-TAGATGCATCAAAATCGGGG
Exon4.3M13F-TGGCTTAAGGAGGAAAAGAGA3786034
M13R-TCTACATCGTGCCTCCATCA
Exon4.4M13F-TTCTGGCTTTCCTGAAATTG3746034
M13R-TAAATCTCGAACAATGGCGA
Exon4.5M13F-TCTGGCCATACTCGTGCATA3296034
M13R-AGCACCTGGGGTAACATCAC
Exon4.6M13F-TCAGGAAGGTCTGATACCCG3536233
M13R-GCACCATTCGTTGATAGGCT
Exon4.7M13F-AAGTGAATTGGCCCTCTCTG4836230
M13R-TGGTTCTGACTCTTCAGGGG
Exon4.8M13F-TTTTGGTAAGCGGCTCCTAA4656235
M13R-TTTCGAGCCTTTTCATGGTC
Exon4.9M13F-TTTCTGCTCTGGAAGGATTC4406230
M13R-TCGTTTACAGCCCTTCTTGG
Exon4.10M13F-TGAACAGAGCCTCCTGGAAT3906232
M13R-CAGCTGGCAAACAGCACTAC
Exon5M13F-CTGATAAAACCCCCAAACGA4036230
M13R-CTGTGTTTGGAAAATGATCACC
Exon6M13F-CCAGTCATAAAAGACCTTTTCC1925634
M13R-GACTGAATGAGAACTTAAGTGGG
Exon7M13F-AAGGTGAAAGTACATTT1196133
M13R-TTCAAATGAGAAGTTTAATG
Exon8/9M13F-CCTTTGAGTTACTTCCTT5736132
M13R-TCATAGTGCATCATCCCTTCC
Exon10M13F-GGAAGGGATGATGCACTATG2546135
M13R-AAGAAAATGGAAAAATGGTCA